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GLP-1 therapeutics are rewriting biopharma—and analytical science is stepping up. 🔬💊In Concentrating on Chromatography, ...
12/06/2026

GLP-1 therapeutics are rewriting biopharma—and analytical science is stepping up. 🔬💊

In Concentrating on Chromatography, Kelly Broster McMahon (Thermo Fisher Scientific) joins David Oliva to unpack how LC‑MS and high‑resolution MS deliver safe, effective GLP‑1 drugs like semaglutide. 🧪

• Why GLP‑1 peptides challenge labs: complex structures, modifications, degradation risk 🧬
• LC‑MS + UHPLC separate look‑alikes, confirm structure, and spot ppb impurities 🔎
• Find sequence variants, oxidation/deamidation, aggregates → protect patients 🛡️
• Scalable workflows from discovery → QC → manufacturing; strong data integrity and compliance ✅🏭
• What’s next: faster separations, multiplexing, AI, automation, PAT, real‑time insights 🚀

• Key takeaway: success isn’t more data—it’s measuring the right attributes earlier, with confidence. 💡

Watch the episode to see how integrated chromatography, HRAM MS, and informatics power biosimilars, equivalence testing, and the next decade of biopharma. 📈

Learn how chromatography, HRAM mass spectrometry, and AI-driven workflows support GLP-1 therapeutics, peptide characterization, and drug development.

• How do cells decide — and how can cancer begin? 🔬🧬• Teams at CEITEC and Masaryk University uncovered a molecular tippi...
11/06/2026

• How do cells decide — and how can cancer begin? 🔬🧬
• Teams at CEITEC and Masaryk University uncovered a molecular tipping point in the Wnt signalling pathway: Dishevelled (DVL) acts as a switch, not a dial. 🎚️
• CK1-driven phosphorylation stacks negative charge on DVL until a threshold flips its shape; DVL then detaches from Frizzled, sending a clear go/no-go signal downstream (Wnt/β-catenin). ⚡
• This on/off logic reframes how tissues coordinate growth and why dysregulation can trigger uncontrolled proliferation. ⚠️
• Understanding these decision points opens avenues to slow or stop tumour‑promoting signals. 🚀

• Published in Science Advances (DOI: 10.1126/sciadv.aed8899). 📖
• Lead scientists: Miroslav Micka (CEITEC); Konstantinos Tripsianes (CEITEC MUNI); Vítězslav Bryja (Faculty of Science, Masaryk University). 👩‍🔬👨‍🔬

Researchers identified a molecular switch in Wnt signalling that regulates cell behaviour and may contribute to understanding cancer development.

🚀 DNA as catalyst is here! Researchers at IOCB Prague led by Edward Curtis unveil deoxyribozyme breakthroughs that make ...
11/06/2026

🚀 DNA as catalyst is here! Researchers at IOCB Prague led by Edward Curtis unveil deoxyribozyme breakthroughs that make sensing faster, cheaper, and smarter 🧬🔬

• Supernova shines 💡: a chemiluminescent deoxyribozyme that glows blue to detect DNA‑editing enzymes. New assays are ~3× faster and >5× cheaper than standard methods ⚡💰
• Smart switching 🧠: configurable “turn‑on” and “turn‑off” sensors enable precise readouts, including for APOBEC3A—accelerating high‑throughput screening and inhibitor discovery 🎯🧪
• Aurora learns 🎇: using artificial evolution + machine learning, the team mapped how four mutations reprogram Aurora’s substrate preference (4‑MUP vs diFMUP) while avoiding pNPP and ELF 🤖📊
• From lab to impact 🌍: robust, low‑cost catalytic DNA tools point to powerful diagnostics, drug discovery, and future personalized medicine.

Backed by two ACS Chem. Biol. 2026 studies by Jakubec, Svoboda, Kurfürst, Svehlova, Veverka, Curtis and by Kráľová, Isler, Volek, Jandová, Kurfürst, Curtis. Institution: Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague).

IOCB Prague researchers advance deoxyribozyme technology for DNA sensing, enzyme screening, and machine learning-guided discovery.

SPE flow stalling? 🧪⚠️ Genevie Hodson, Senior Manager of Technical Support and Applications at Phenomenex, shares the to...
10/06/2026

SPE flow stalling? 🧪⚠️ Genevie Hodson, Senior Manager of Technical Support and Applications at Phenomenex, shares the top culprits and quick fixes.

• 🧪 Viscous/cloudy samples: Dilute, centrifuge, filter—or use larger particles (Strata-X 33 µm; Strata XL 100 µm).
• ⚙️ Low manifold pressure: Check the SPE manifold, instrumentation, and individual positions; often appears in conditioning/blanks.
• 🌊 Immiscible solvents: Avoid non-miscible pairs (e.g., hexane + water); issues show up during conditioning/blanks.
• 🧬 Precipitation/proteins: After loading, clogs can form; dilute, reduce volume, avoid denaturing solvents, and add a low ionic strength buffer wash.

• 🔍 Tip: Note exactly when the flow stalls, then change one variable at a time to pinpoint the root cause and restore performance fast.

Troubleshoot SPE flow problems by identifying common causes such as viscous samples, manifold pressure issues, solvent incompatibility, and precipitation.

Small cap, big impact in HPLC 🔬💧 KNAUER shows how choosing crimp, screw, or snap caps can make or break sample integrity...
10/06/2026

Small cap, big impact in HPLC 🔬💧 KNAUER shows how choosing crimp, screw, or snap caps can make or break sample integrity, evaporation control, and workflow speed.

• Crimp caps 🔒🛡️: Maximum airtight sealing; best for volatile compounds, headspace GC, sensitive LC‑MS/MS, and long‑term storage.
• Screw caps 🔁⚙️: Tool‑free, re‑sealable convenience; ideal for routine HPLC, repeated access, and broad autosampler compatibility.
• Snap caps ⚡⏱️: Fastest handling for high‑throughput; suited to stable, non‑volatile analytes and short‑term storage.

Key takeaway ✅: Match sealing performance to analyte volatility, storage duration, and instrument compatibility. The right cap reduces evaporation and contamination while boosting reproducibility and throughput.

Looking to protect samples and streamline prep? Explore KNAUER originals HPLC Vials.

Compare crimp, screw, and snap caps for chromatography. Discover their strengths, limitations, and best applications for reliable sample protection.

New in the LabRulezLCMS Library — Week 24, 2026! 🚀 Our repository keeps growing with high-impact resources across HPLC, ...
10/06/2026

New in the LabRulezLCMS Library — Week 24, 2026! 🚀 Our repository keeps growing with high-impact resources across HPLC, LC/MS, IC, and IMS-MS. 🔬

• Agilent Technologies: Q-TOF MS/MS confirms sequences of GLP-1 agonists (liraglutide, tirzepatide, retatrutide) with complete coverage using Revident LC/Q-TOF and MassHunter BioConfirm. 🧬
• KNAUER: HPLC-UV oligonucleotide analysis—TEA/HAc rivals TEA/HFIP;

This week we bring you application notes by Agilent Technologies, KNAUER and Shimadzu, other document by Thermo Scientific, poster by Waters Corporation/ASMS.

Make your PT results work for you! 🧪📊 Eurachem explains how providers set assigned values, uncertainty, and dispersion—s...
09/06/2026

Make your PT results work for you! 🧪📊 Eurachem explains how providers set assigned values, uncertainty, and dispersion—so labs can interpret proficiency testing correctly.

• 🔍 Selection tip: The Eurachem Guide (eds. B. Brookman & I. Mann) urges you to review a PT provider’s statistical approach before you join.
• 📍 Location (xpt): Providers may use mean, median, or robust estimators. ISO 13528:2022 recommends robust methods when outliers or asymmetry exist. Small datasets can make any estimator unreliable. Different analytical methods may get method-specific locations. A gap vs. a reference can signal method bias.
• 📈 Dispersion (σpt): Standard deviation or robust measures (MADe, nIQR, Algorithm A, Qn, Q). Reported in measurand units or %. Classical SD usually follows outlier removal. Beware small N.
• 📏 Uncertainty u(xpt): For the arithmetic mean, calculate as s divided by sqrt(p). For robust estimators, ISO 13528 suggests a conservative 1.25 multiplier with s*.

• ✅ Takeaway: Know how xpt, u(xpt), and σpt are derived—your conclusions depend on it.

Understand the key statistical parameters used in proficiency testing schemes, including assigned values, uncertainty, and dispersion estimates.

⏳ Early Bird + abstracts close June 15, 2026 for the 19th CSSC & MSMS!• 📍 Faculty of Science, Palacký University Olomouc...
09/06/2026

⏳ Early Bird + abstracts close June 15, 2026 for the 19th CSSC & MSMS!

• 📍 Faculty of Science, Palacký University Olomouc (Czech Republic)
• 👩‍🔬 For researchers, students, instrument specialists and industry
• 🎯 Topics: atomic/molecular spectrometry; IR/Raman (incl. SERS); XRF; mass spectrometry & hyphenated techniques; laser spectroscopy; statistics & metrology; Mössbauer applications
• 🎁 Included: program, e-abstract book, lunches/coffee, excursion, Welcome Party & Gala Dinner
• 💸 Save now: Early rates for IMMSS and SSS members, students, 1‑day and accompanying persons
• 📝 Abstracts: EN; A0 posters (portrait); talks 20/30 min

• 👉 Register: https://cssc2026.spektroskopie.cz/registration_online
• 👉 Submit abstract: https://cssc2026.spektroskopie.cz/abstract_submission_online

🔬 Join Central Europe’s leading event in spectroscopy and spectrometry!

Early Bird registration and abstract submission for the 19th Czech-Slovak Spectroscopic Conference close on June 15, 2026. Register today.

🧬 mAb manufacturing, made simple. Maria Gruebner (Thermo Fisher Scientific) reveals how switchable 2D‑LC‑UV‑MS puts CQAs...
09/06/2026

🧬 mAb manufacturing, made simple. Maria Gruebner (Thermo Fisher Scientific) reveals how switchable 2D‑LC‑UV‑MS puts CQAs under near real‑time control.

• 🧪 1D ProA cleanup, then toggle: SCX for charge or SEC for size—no replumbing.
• 📈 UVD for rapid trendlines; 🧠 HRAM‑MS for intact‑level insights into glycoforms, modifications, and aggregates.
• ⏱️ At‑line in ~1 h from bioreactor to insight; catch glycation, deamidation, drift, and aggregation early.
• 💸 Cut scrap, protect yield, avoid restarts; standardized AQbD workflow eases transfer and scale‑up.

Outcome: fewer surprises, faster course corrections, and steadier biotherapeutics. ✅

Switchable 2D-LC-UV-MS workflows enable rapid monitoring of mAb quality attributes and support timely process interventions.

Reality check for “female desire enhancers” 🚨 A new ACS Omega study develops a fast, sensitive LC–MS/MS assay for fliban...
08/06/2026

Reality check for “female desire enhancers” 🚨 A new ACS Omega study develops a fast, sensitive LC–MS/MS assay for flibanserin and puts 15 Türkiye-market products to the test.

• 🔬 Method: ICH-validated LC–MS/MS with 5‑minute runtime and ng/mL LOQ; findings confirmed by LC‑MS‑IT‑TOF
• 📦 Market scan: Most products contained no or only trace flibanserin; labels often mismatched contents and some lacked manufacturer details
• ☕ What was inside: caffeine, lactose, and citric acid—ingredients with no therapeutic effect for HSDD
• 🛡️ Implications: authenticity, efficacy, and consumer safety concerns for OTC “enhancers”
• ♻️ Sustainability: strong green/white/blue profile (AGREE, WAC, BAGI, ComplexMoGAPI), enabling routine QC use
• 👩‍🔬 Authors: Abeer Elriş, Mazlum Akif Altun, Saniye Özcan, Serkan Levent, Nafiz Öncü Can

Bottom line: a robust, rapid, and eco-conscious workflow to safeguard women’s health and trust—ready for QC labs, supplement testing, and regulatory surveillance.

A validated LC-MS/MS method detects flibanserin in female sexual enhancer products, revealing absent or very low levels.

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